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    Livecyte Applications

  • Livecyte measures individual cell growth independently from proliferation.
  • Cell growth is more complex than just cells multiplying
    • They can increase in size without dividing, they can divide asymmetrically, they can grow to a certain size then stop.  All these aspects of cell growth are lost with most live cell assays.  Not with Livecyte.

Measure true cell growth at the single cell level

  • Directly measure cell motility with individual cells
  • Separate out migration from proliferation in a single experiment
  • Observe differences between leading edge and following cells
  • Correlate motility to morphological differences

Go beyond traditional wound healing assays

  • Livecyte automatically tracks cells, even in a 96-well plate.
  • Just seed your cells, monitor with Livecyte and a full range of cell migration parameters will be generated.
  • Compare the speed, directionality, confinement and heterogeneity of different populations.

Automatically measure cell motility without a scratch wound assay

  • Directly measure mitotic time with individual cells
  • Measure random and chemotactic migration
  • Identify and characterise differences in subpopulations in  heterogenous primary cultures
  • Correlate motility to morphological differences
  • Generate statistically valid results from multiple repeats in 96-wells

Automatically measure the morphology and motion of every cell

  • True label-free cell count, not just confluence
  • Monitor dry mass to automatically determine viability and cell death
  • Statistically robust results from multiple repeats in 96-well plates
  • Generate label-free dose-response curves
  • Identify dynamic toxic responses missed by other methods

Go beyond confluence-based toxicity assays

  • Label-free timelapse tube formation assays
  • High-resolution large fields-of-view with no stitching
  • Perform assays on Matrigel and standard plates
  • Automated post-acquisition focus ensures consistent image quality for every well
  • Simple, yet powerful analysis of tube formation metrics with Application Dashboard outputs

Automated analysis to determine tube length, formation, diameter etc

  • Identify morphological phenotypes
  • Watch and quantify cell differentiation
  • Monitor stem cell populations, for many days, without perturbing cell behaviour
  • Image through plastic and coated plates
  • Image on extracellular matrices
  • Cells are still viable after imaging

Obtain quantitative measurements without perturbing your cells